RNA Binding Protein (RBP)

An Evo-Devo approach to the role of RNA-RBP binding on Alternative Splicing – EvoRNAs
The EvoRNAs project aims to leverage the evolution of RNA-RNA Binding Protein (RBP) interactions and machine learning to predict RNA recognition rules of two highly conserved key RBPs and their interacting lncRNAs and mRNAs, to assess the impact on the generation of splicing diversity across evolutionary distant species.
Jul 15, 2022 ANR
ANR
An Evo-Devo approach to the role of RNA-RBP binding on Alternative Splicing – EvoRNAs
Axis 1: Building a model to predict function and mode of action of lncRNAs in Arabidopsis and Tomato

Using the molecular characteristics of lncRNAs’ mode of action in the control of transcription through chromatin remodeling 2,4 or splicing 3,5 we were already able to uncover lncRNAs acting similarly. We now want to extend this approach to cluster lncRNAs with common modes of action and build a predictive model integrating specific molecular and sequence features.

Jul 15, 2022
Axis 2: Role of lncRNAs in heat stress response in Arabidopsis and tomato

Using time-course transcriptomic data along thermotolerance acquisition and heat shock, we will reconstruct co-expression modules and the gene regulatory network (GRN), including AS regulation, of heat stress acclimation in Arabidopsis and tomato.

Jul 15, 2022
Axis 3: Contribution of lncRNAs to temperature adaptation.

We will pursue functional studies on Arabidopsis lncRNAs isolated in previous screens and on heat stress associated lncRNAs from axis 2 to better understand their regulatory function, mode of action and biological functions.

Jul 15, 2022